LSM 880 with Airyscan/ LSM880 NLO
Zeiss LSM 880 雷射共軛焦顯微鏡


Revolutionize Your Confocal Imaging
領先世界,唯一兼具快速、高靈敏度、超高解析度的共軛焦顯微鏡

●高解析:提升1.7倍的解析度(逼近超高解析度SIM的解析能力)
●高速掃描:512X512 每秒13 幅(同時增加三成的靈敏度)
●高靈敏度: 高感度32個磷砷化鎵GaAsP感測器陣列(超越共軛焦顯微鏡的極限)

您的螢光樣品因為太小看不清楚,或是移動過快無法及時記錄動態的過程,或是螢光漂白影響實驗結果,針對活體細胞與螢光弱的樣品,Zeiss 推出最新技術Airyscan,依照樣品與實驗的需求,可以自由選擇增加靈敏度同時提升1.7倍的解析度,488nm波長的激發下,XY可以解析到140nm,Z軸也有400nm的解析力,或選擇高解析度突破一般雷射共軛焦顯微鏡,亦可以選擇提高靈敏度來加快擷取影像的速度,讓您的實驗達到完美極致!

Carl Zeiss 保留原先LSM780高感度GaAsP感測器,是傳統PMT的兩倍以上, 以及光譜解析的特性,一次掃圖立即可獲得螢光放光光譜訊號

掃圖一次即可得到完整的螢光光譜圖!

HeLa cells, Courtesy of S. Traikov, BIOTEC, TU Dresden, Germany
Fixed tumor cells, Courtesy of P. O`Toole and P. Pryor, University of York, UK
Sample courtesy of Michael W. Davidson, The Florida State University African green monkey kidney TOMM20 (AF 568), Tubulin (AF 488) and DAPI Comparing confocal with Airyscan, 3D
COPYRIGHT: Cell line provided by Jan Ellenberg, EMBL, Heidelberg. Mitosis in HeLa-Kyoto cell line, imaged with ZEISS LSM 880 with Airyscan. Video showing Histone 2B (H2B, red, mCherry) and microtubule end binding protein 3 (EB3, green, EGFP) during mitosis.
Drosophila Retina Airyscan 3D - Maximum intensity projection
Skin tissue from pig labelled with Ethyleneblue

Spectral tile scan

Zeiss LSM 880 with airysacn

White Paper: Airyscanning - Short version

Technology Note: The Airyscan Detector from ZEISS

Airyscan with Fast Mode - Introductory White Paper

Airyscan in Fast Acquisition Mode

★產品特性

4倍的掃描速率,512x512 pixel 19fps

1.5倍的解析度,x=145nm, y=180nm, z=450nm

4倍的靈敏度提升

Resonance Scanner – 80 FPS

Slowed to 8.3 fps

Airyscan Fast Mode – 96 FPS

Slowed to 10.0 fps

Cardiomyocyte Cells with tubulin-EMTB to measure microtubule buckling, Cells pulsed @ 1 Hz, Samples Courtesy of Ben Prosser
 

Standard Confocal – 15 FPS Pixel Dwell 320ns

Airyscan Fast Mode – 30 FPS Pixel Dwell 580ns

Color Coded Time Projection

Cardiomyocyte Cells Loaded with Fluo4

Images and Samples  Courtesy of Ben Prosser, UPENN

LSM, GaAsP

Fast mode, SR

Airyscan, SR

 

Arabidopsis thaliana root

Golgi: RFP in magenta for movie

Samples provided by Professor C Hawes, Oxford Brookes University, UK

 
 
 

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