ZEISS LSM 800 with Airyscan Your Compact Confocal

高規格經濟實惠的雷射共軛焦顯微鏡

Scan head 全新光路設計

VSD beamsplitter提供全方位的訊號擷取波段彈性,可調精度達1nm,不再受限於傳統的濾片限制,可以收集更多訊號。
精準的Pinhole位置,絕無色差問題,可連續調整針孔大小,進而達到精確的光學切片厚度。
FixGate main beamsplitter,操作方便,且採與LSM 710相同的低角度設計,高反射性的樣品也不會干擾影像擷取。

高解析:提升1.7倍的解析度(逼近超高解析度SIM的解析能力)

高靈敏度: 高感度32個磷砷化鎵GaAsP感測器陣列(超越共軛焦顯微鏡的極限)
低價格高規格的組合,客製化滿足不同的實驗需求

 

LSM 800 延續 LSM 700的分光設計

VSD beamsplitter提供全方位的訊號擷取波段彈性,可調精度達1nm,不再受限於傳統的濾片限制,可以收集更多訊號。
精準的Pinhole位置,絕無色差問題,可連續調整針孔大小,進而達到精確的光學切片厚度。
FixGate main beamsplitter,操作方便,低角度設計,高反射性的樣品也不會干擾影像擷取。 

實用的雷射模組

提供多支激發雷射波長選擇(405, 488, 561 and 640nm), 適合一般實驗室常使用的染劑及螢光蛋白觀察。
固態雷射具有體積小、穩定等優點,且壽命超長,省去日後需常更換的煩惱。

經濟實惠的價格

在您有限的預算內,LSM 800以親切的價格提供精準的共軛焦光學設計與ZEISS精湛的光學品質!
簡易親切的操作方式與多功能用途的軟體介面 ZEN 2
整體雷射模組與電腦控制系統體積大幅減小,即使在小型實驗室當中也方便設置。
 

螢光感測器可以選擇傳統光電倍增管(PMT)或是磷酸砷化鎵(GaAsP)感測器

LSM800 同時支援Zeiss 推出最新技術Airyscan! ,XYZ解析度同步提升1.7倍的超高解析技術,488nm波長的激發下,XY可以解析到140nm,Z軸也有400nm的解析力!
圖說: Mouse brain slice; EGFP-Thy1 (green): nerve cells (subset), Calretin-Cy3 (red): Calretinin-expressing neurons, GAD65-Cy5 (blue): GABAergic synapses. Scale bar 50 µm.
 
圖說: Living Pig Kidney Epithelial cells (LLC-PK1), green: Tubulin-eGFP, red: h2b-mCherry; Imaged with ZEISS LSM 800 with Airyscan, Plan-Apochromat 63x/1.4 Oil.
 
圖說: Drosophila brain, neuromuscular junction stained for Bruchpilot (BRP). LSM 800 application image, comparison between confocal LSM and Airyscan.
 
圖說: Comparison between confocal and Airyscan image. HeLa cells, red: Mitochondria membran, green: microtubuli, magenta: Actin fibres; acquired with LSM 800. Scale bar 2 µm.
 
圖說: 
The example shows Kaede expressed in HepG2 cells before photoactivation (0 s) and at different time points (1 s, 3 s and 10 s) after repeated photoactivation (every 0.1 s) with 405 nm at the indicated regions (white box).
 Kaede diffuses freely between the nucleus and the cytoplasm. The relative intensities in AU of the non-converted form (green bars) and converted form (red bars) are shown in each image.
 
圖說: 
Hep G2 cells, LSM 800 application image for FRET, Two interacting proteins (donor false colored in green, acceptor false colored in red) in HepG2 cells ("before bleach"). By acceptor-photobleaching (within the indicated white circle) acceptor intensity will decrease while donor intensity will increase ("after bleach") as indicated by the green (donor) and red (acceptor) bars. The increase in donor intensity can be used to calculate FRET efficiencies.

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