Zeiss Lightsheet  7 層光激發活體培養&澄清化組織顯微鏡


The first commercial Light Sheet Fluorescence Microscope for 3D Multiview Imaging of Living and Cleared Specimens.
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  1. 活體培養: 溫控加熱或降溫,固定CO2 濃度,樣品浸泡在培養液中
  2. 澄清化組織: 澄清化組織: 支援各式折射係數1.33~1.58的澄清化溶劑
  3. 快速觀察: 速度媲美Spinning Disc,30 fps
  4. Multiview 3D影像:多角度影像重組3D全貌
  5. 光毒害低: Phototoxic & photo-bleaching低於雷射共軛焦顯微鏡,雷射光只激發焦平面
  6. Lightsheet 專有的Pivot Scan: 焦平面上快速掃描,避免影像上的陰影產生
  7. Fast Z Stack acquisitions: 快速準確的掃描Z Stack
  8. 適合不同尺寸的樣品(μm~mm): 激發深度可逼近雙光子雷射的穿透力
  9. 雷射選擇性多,可同時支援六支,適合不同螢光樣品: 405nm, 445nm, 488nm, 515nm, 561nm, 638nm

Lightsheet原理介紹:

激發與接收訊號光路分開,側光激發樣本,90度接收訊號,提升側面的穿透度與解析度,樣品可從微米到毫米的尺寸,彌補一般共軛焦顯微鏡的樣品限制。
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Lightsheet-7_LSFM_illumination-principle
 
動畫介紹光路與操作

樣品製備介紹


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Lightsheet 應用

  • Morphogenesis and embryogenesis in developmental biology and systems biology (embryogenesis of Drosophila, Zebrafish and other model organisms)
  • Organogenesis and cell dynamics (cardiac development, blood flow, angiogenesis and vascular development, neuro-development by calcium imaging)
  • 3D cell culture (spheroids and cysts, tissue culture, organotypic cultures)
  • Imaging of marine organisms (ciona, squid, plankton, flat worms, etc.)
  • Fluorescence imaging of structural features of large (mm-sized) specimens (early mouse embryos, Zebrafish & Medaka Fish)
  • Imaging of optically cleared specimens (tissue sections, mouse brain, embryos, organs, spheroids and biopsies) that are optically cleared with almost any of the common clearing media with a refractive index between n = 1.33 (water) up to n = 1.58.
 

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2 days old living zebrafish heart

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Marine amphipod Parhyale hawaiensis

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Clarity Easyindex HCBI mouse brain Image

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Live imaging of Drosophila embryo


Lightsheet movies:
Zebrafish Heart Development
Zebrafish Embryo Development
Arabidopsis root growth
Tribolium embryo development

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