Zeiss Lightsheet 7 層光激發活體培養&澄清化組織顯微鏡
The first commercial Light Sheet Fluorescence Microscope for 3D Multiview Imaging of Living and Cleared Specimens.
- 活體培養: 溫控加熱或降溫，固定CO2 濃度，樣品浸泡在培養液中
- 澄清化組織: 澄清化組織: 支援各式折射係數1.33~1.58的澄清化溶劑
- 快速觀察: 速度媲美Spinning Disc，30 fps
- Multiview 3D影像:多角度影像重組3D全貌
- 光毒害低: Phototoxic & photo-bleaching低於雷射共軛焦顯微鏡，雷射光只激發焦平面
- Lightsheet 專有的Pivot Scan: 焦平面上快速掃描，避免影像上的陰影產生
- Fast Z Stack acquisitions: 快速準確的掃描Z Stack
- 適合不同尺寸的樣品(μm~mm): 激發深度可逼近雙光子雷射的穿透力
- 雷射選擇性多，可同時支援六支，適合不同螢光樣品: 405nm, 445nm, 488nm, 515nm, 561nm, 638nm
Lightsheet原理介紹：
激發與接收訊號光路分開，側光激發樣本，90度接收訊號，提升側面的穿透度與解析度，樣品可從微米到毫米的尺寸，彌補一般共軛焦顯微鏡的樣品限制。
Lightsheet-7_LSFM_illumination-principle
動畫介紹光路與操作
樣品製備介紹
Lightsheet 應用
- Morphogenesis and embryogenesis in developmental biology and systems biology (embryogenesis of Drosophila, Zebrafish and other model organisms)
- Organogenesis and cell dynamics (cardiac development, blood flow, angiogenesis and vascular development, neuro-development by calcium imaging)
- 3D cell culture (spheroids and cysts, tissue culture, organotypic cultures)
- Imaging of marine organisms (ciona, squid, plankton, flat worms, etc.)
- Fluorescence imaging of structural features of large (mm-sized) specimens (early mouse embryos, Zebrafish & Medaka Fish)
- Imaging of optically cleared specimens (tissue sections, mouse brain, embryos, organs, spheroids and biopsies) that are optically cleared with almost any of the common clearing media with a refractive index between n = 1.33 (water) up to n = 1.58.
2 days old living zebrafish heart
Marine amphipod Parhyale hawaiensis
Clarity Easyindex HCBI mouse brain Image
Live imaging of Drosophila embryo
Lightsheet movies：
Zebrafish Heart Development
Zebrafish Embryo Development
Arabidopsis root growth
Tribolium embryo development