Zeiss Lightsheet Z.1 層光顯微影像技術
The first commercial Light Sheet Fluorescence Microscope for long term live 3D imaging Lightsheet Z.1-Fast, Gentle Imaging for Living Samples
活體培養: 溫控加熱或降溫，固定CO2 濃度，樣品浸泡在培養液中。
快速觀察: 速度媲美Spinning Disc，30 fps。
Multiview 3D影像:多角度影像重組3D全貌。
光毒害低: Phototoxic & photo-bleaching低於雷射共軛焦顯微鏡，雷射光只激發焦平面。
Light sheet 專有的Pivot Scan: 焦平面上快速掃描，避免影像上的陰影產生。
Fast Z Stack acquisitions: 快速準確的掃描Z Stack。
適合不同尺寸的樣品(μm~mm): 激發深度可逼近雙光子雷射的穿透力。
雷射選擇性多，可同時支援六支，適合不同螢光樣品: 405nm, 445nm, 488nm, 515nm, 561nm, 638nm
Light sheet原理介紹
激發與接收訊號光路分開，側光激發樣本，90度接收訊號，提升側面的穿透度與解析度，樣品可從微米到毫米的尺寸，彌補一般共軛焦顯微鏡的樣品限制。
Figure from the PhD thesis of Jörg Ritter (2011),University of Bonn, Germany)Multiview Imaging
轉動樣品，多個角度觀察樣本，軟體重組成3D影像全貌
Real Life 活體培養
Light sheet 應用
Morphogenesis and embryogenesis in developmental biology and systems biology (embryogenesis of Drosophila, Zebrafish and other model organisms)
Organogenesis and cell dynamics (cardiac development, blood flow, angiogenesis and vascular development, neuro-development by calcium imaging)
3D cell culture (spheroids and cysts, tissue culture, organotypic cultures)
Imaging of marine organisms (ciona, squid, plankton, flat worms, etc.)
Fluorescence imaging of structural features of large (mm-sized) specimens (early mouse embryos, Zebrafish & Medaka Fish)
Lightsheet Z1 Movie
Zebrafish Heart Development
Zebrafish Embryo Development