Zeiss Elyra S.1 超解析螢光顯微鏡

• SIM_Structured Illimination Microscopy

• 真正的3D Super resolution!

• 不需要特殊的螢光染劑/蛋白

ELYRA S.1硬體搭配特色
• turnkey SR-SIM system 
• 405 nm, 488 nm, 561 nm and 642 nm and most of your standard dyes
• multiple gratings to achieve uncompromised resolution gain for different wavelengths
• excellent color correction
• a Piezo stage insert for fast, reliable and reproducible Z-sampling
• a sensitive EM-CCD camera for wide-field detection (Andor iXon series)
• incubation for highest stability
• wide-field and structured illumination on one system
• specific gratings for each excitation wavelength, motorized exchange for multicolor 　experiments 
• choice between 3 or 5 pattern rotations for optimized speed or maximal resolution 
• dual camera option for simultaneous two color imaging
 

Software of ELYRA S.1 軟體特色
• all superrresolution features are integrated into the ZEN software 
• 64 bit architecture 
• batch processing for multiple files 
• advanced algorithm with automatic and professional mode for superresolution image 　reconstruction 
• supports use of positional fiducials for automatic drift correction 
• supports system specific chromatic aberration correction for reliable colocalization 　analysis
　

原理說明

光路中利用有固定空間頻率的柵格（gird）投影在樣品上形成Moiré pattern，利用所得到的影像可以利用軟體計算出未知樣品真實影像。