Zeiss InTune 可見光Tunable Pulse Laser 共軛焦顯微鏡專用 

In Tune with LSM 710/LSM 780

  • No background issues
  • Good stability
  • Good choice for multi-fluorescence imaging
  • Takes full advantage of the LSM710 / 780 benefits:
    High SNR and High sensitivity
  • Full freedom of choice for laser lines FLIM capable
  • Fully integrates into 710 / 780 system concept“ just another laser”
  • LSM 710& LSM 780新雷射系統選擇  
  • 與LSM 710/780 QUASAR detecteor為最佳搭配:同時擁有最高的訊號激發excitation選擇與emission擷取彈性 
  • In Tune的可選範圍為488~640nm,平均功率Power > 1,5 mW 
  • 頻率為40 MHz、每次脈衝 < 5 ps,非常適於FLIM的實驗,比同等級的白光雷射雜訊更低,data更為準確!  
  • FILM感測器領導品牌Becker-Hickl的DR. Wolfgang Becker強力推薦!
  • Specifications:

Wavelength range

488 –640 nm

Average power

> 1.5 mWat the set wavelength

Repetition rate

40 MHz

Pulse length

< 5 ps

Tuning speed

< 50 nm / s

Bandwidth

< 3 nm

  • 與他廠牌白光雷射之比較:
LEICA SP5 X
ZEISS LSM 710 with In Tune

不能與其他雷射共用

可與其他支雷射(例如561, 594nm laser)共用

40-80 MHz

40 MHz –
比白光雷射的80MHz更適於FLIM檢測實驗; 且完全整合至LSM 軟體ZEN當中使用相當簡單方便

1-3 mWper nm; 通過AOBS之後可能銳減而<1.5mW

> 1.5 mW; out of fibre, AOTF already passed; only needs to go through MBS

雜訊較高Laser “noise”as the white light needs to be suppressed; might lead to unwanted cross-excitation or background in the image

影像背景雜訊低,“Clean”images, no potential cross excitation; image quality as high as when using standard lasers on the LSM 710

手動調整波長才能進行Excitation Fingerprinting

Excitation Fingerprinting implemented with Macro,簡單設定軟體即會自動完成

★In Tune相較於 White-light laser與 diode lasers的優異點

  • Spectral line:
    No side lopes, no higher order filter transmission, suppression of other wavelengths > 60 dB-

  • Pulse shape:
    No secondary pulses, no after-pulsing or ringing-

  • Polarization:
    Laser emission obtained under well controlled polarization conditions, polarization ratio better than > 100:1, low polarization noise-

  • Stability (low noise):
    High due to stable frequency conversion process and implemented self-calibration procedures
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