Fast Evaluation of Standard Elispot Specimen

Efficient and reproducible method for Enzyme Linked Immuno SPOT assay evaluations.
Complete package for immediate high speed measurements with high resolution Re-evaluation of stored well images.

The specimens will be measured using the KS ELISPOT compact system under a stereo microscope with incident illumination, and equipped with a motor stage. The images of the cytokine spots are taken with a true color camera. Following digitization the KS ELISPOT software proceeds with automatic data processing.

Fast evaluation times are obtained by acquiring just one image per well. The measurement time for a complete plate is less than 5 minutes. A reliable identification, even of smaller spots is possible by using the scanning mode of the system to generate a higher primary magnification image. As an alternative a high resolution digital color camera Axiocam MRc may be used. An upgrade to the high-resolution image KS ELISPOT is possible.

Feature

•  連控電動載物台與數位相機 , 使實驗更有效率的進行
•  可進行背景校正 , 使點數計算更為方便準確
•  高精準性的免疫反應點數判別法 , 利用許多影像參數如直徑、色調、飽和度、對比度、形狀與斜率等自動判別有效點數並能有效的去除因染色造成的無效點數
•  軟體可自動縫合在高倍數物鏡所擷取的多張影像 , 並將它們轉換成單張影像 , 因此可在高解析力下判別反應點數
•  軟體內建各種分析統計數據報告每一個單點的數據包含大小、強度等 個別細胞盤 (well) 統計數據包含面積、點數、顏色深度等  

 

KS ELISPOT : http://www.zeiss.de/C12567BE0045ACF1?Open