螢光共定位分析
Meaningful results of protein colocalization & simple handling

ZEISS軟體ZEN提供一個螢光共定位(colocalization)分析功能,判斷染色樣本上的兩種螢光蛋白染色位置是否於同一區域或者兩個螢光蛋白在空間位置上並不具有相關關係,不需經由其他軟體和轉檔即可在ZEN軟體上做影像的分析,並且在結果上提供了許多相關數據的呈現。
更詳細的螢光共定位軟體操作分析影片(Colocalization Walkthrough)
https://www.zeiss.com/microscopy/us/local/zen-knowledge-base/zen.html
文章來源: microscopy@zeiss.com

Use the Colocalization module to analyze the degree of overlap between two different fluorescence channels. To do this, the ZEN module employs established colocalization methods, such as the automated threshold value method of Costes, and the coefficients according to Pearsons and Manders. Obtain meaningful results on the colocalization of proteins as a scatter plot, image, and data table.
If you want to analyze certain areas of the image, simply mark the corresponding region directly in the image and study the scatter plot. ZEN then supplies the data both for the whole image and the selected regions.

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Histone-2B-expressing HeLa cells, transfected with a cytoplasmically localized mutant of the HIV protein Rev. The figure shows two-time points, before and after treatment. The scatter plot clearly shows the increase in the colocalization of histone-RFP and Rev-GFP. Cell cultures: Prof. Ruth Brack-Werner.

Highlights
Quick quantitative information Explore the amount of colocalization of two labeled structures in two channels of a multi-channel fluorescence image
Image processing
Extract colocalizing areas as new images for further processing
Reliable measurements
Obtain objective measurements of colocalization, independent of display parameters
Ease of use
Receive great results with simple steps through easy to use tools

Upgrade your ZEISS microscope
Upgrade your ZEISS microscope with Colocalization option and benefit from meaningful results of protein colocalization.
As technical requirements may apply on some systems, please contact us to learn more about Colocalization and how your imaging will benefit from an upgrade: microscopy@zeiss.com
 
Colocalization for ZEISS ZEN Imaging Software
Meaningful results of protein colocalization

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Colocalization-tool with ZEN (blue edition).

 
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Colocalization-tab with ZEN (blue edition).

 

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