Nikon Ti2 研究級倒立顯微鏡(全系列)
Nikon Eclipse Ti2提供了前所未見的大視野範圍(Field of view=25mm)，讓您的科研級sCMOS相機視野範圍不再大材小用，且讓高通量擷取影像更快速。因應對超高解析影像 (Super resolution)的顯微鏡需求，Nikon Ti2更特別加強了穩定性，降低影像飄移(drift)，且大幅提升了軟體對於控制硬體的功能，來達到高速影像的要求。另外Nikon Ti2獨特的智能感知導引功能，透過圖像化的介面，當操作錯誤時能及時提醒使用者。為了實驗的重現性及品質，每個感知元件皆能在擷取影像中自動記錄，再搭配Nikon強大的擷取及分析軟體Nikon NIS-Elements，全新革命性的影像體驗就此展開。
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★Nikon Ti2研究級倒立顯微鏡擁有三種機型：
◆Ti2-U手動機型 ◆Ti2-A手動智能感知 ◆Ti2-E電動智能感知
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★Nikon Ti2研究級倒立顯微鏡產品優勢：
Ａ.突破性的視野範圍：
當市面上的大片幅sCMOS影像擷取系統陸續推出，傳統的18mm視野範圍無法放入所有範圍的影像而犧牲掉邊界的影像，且影像邊緣會出現暗角。為了改進上述缺點，Nikon推出Ti2系列，使用者可真正利用空前的25mm拍照大視野範圍，提升了觀察尺度。另外，較大的視野讓您花較少的時間，提升了拼圖速度，滿足更快的影像擷取以及高通量分析的需求。
▲Single FOV of cultured neurons, captured with a CFI Plan Apo lambda 60x objective and DS-Qi2 camera.
Photo courtesy of J. Rappoport, Nikon Imaging Center, Northwestern Univ.; Sample courtesy of S. Kemal, B. Wang, and R. Vassar, Northwestern Univ.
A-1: 提升全視野的明亮度
在拍攝高倍DIC或是拼圖的過程中，是否曾受到亮度不均而感到困擾？
Nikon Ti2搭配獨家專利Fly-eye技術，提供高功率的LED燈源，確保在大視野範圍觀察下的亮度平均。
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螢光的大視野範圍觀察也兼顧到了亮度，Ti2可選購搭配石英fly-eye鏡片，提供全波段光譜包含紫外光更高的穿透率。
更大直徑的螢光濾片組，加厚鍍膜的濾片，提高更好的訊雜比。
 大視野螢光光源
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 大尺寸螢光濾片
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Ａ-2. 直徑加大的觀察模式
為了因應25mm大視野的成像孔洞，觀察光路直徑也隨之增加。比起傳統顯微鏡幾乎可增加2倍的視野範圍，讓使用者搭配大感光元件CMOS時能有最佳的表現。
 增擴直徑後的光路鏡片
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 超大視野範圍25mm的分光孔
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Ａ-3. 提供更大視野的物鏡組
Nikon Apo的物鏡有優異的平場校正功能提供高品質的影像，Apo OFN25系列提供較大的視野範圍讓使用者能更快獲得影像。
*OFN 25=Objective Field Number 25，可搭配視野範圍最大為25mm的目鏡
Ａ-4. 大視野相機
Nikon擁有多年單眼數位相機的研發技術經驗，提供1625萬畫素CMOS感光元件的大片幅相機，能完美搭配Ti2的25mm的視野範圍。
 DS-Qi2 單色高感光螢光相機
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 DS-Ri2 彩色高速相機
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Ｂ.出類拔萃的Nikon光學系統
Nikon的高精確性CFI60無限遠光學系統，帶給使用者更清晰的影像。
B-1. Apodized Phase Contrast 相位差模式
Nikon獨有的相位差模式，相較於傳統相位差效果，更加強對比及減少假光。
▲BSC-1 cells captured with CFI S Plan Fluor ELWD ADM 40xC objective
▲裝置在APC物鏡的相位環示意圖
B-2 DIC 微分干涉相位差
Nikon考量使用DIC影像需要高解析度和高對比的可見光細胞表面影像，因此設計出每顆DIC物鏡皆有搭配專屬的DIC模組片，達到最高品質的效果。
Ｂ-3 NAMC (Nikon Advanced Modulation Contrast)
Nikon獨有技術可用塑膠底容器即可帶有立體感成像，尤其是透明樣本及卵子。NAMC可提供3D的立體效果，對比度則可簡單的被調整。
NAMC/20x
B-4 螢光影像技術
Nikon λ系列物鏡使用奈米晶體鍍膜技術，降低光線的反射，提高螢光的穿透率。對於低螢光訊號的樣本和需多色螢光激發的樣本的觀察有極大幫助。再搭配Ti2新型的濾片，對於目前單分子影像及光激發應用實驗上更有強化效果。
C：Intelligent Assist Guide 智能協助指引 (Ti2-E/A)
有了智能協助指引幫助使用者無須記住冗長的校正及操作步驟，減少使用上的錯誤，維持實驗的再現性。
C-1 顯微鏡狀態的連續展現
透過內建的感知偵測器，所有當您在擷取影像的資訊皆被記錄下來，因此可以輕鬆地得知實驗操作的參數及操作錯誤通知。除此之外，內建感知的照相機可提供相位差、DIC、雷射校正引導。
▲發光面板方便於暗室使用
C-2 操作引導精靈
實驗進行中但顯微鏡有校正或操作問題怎麼辦？
新的Ti2提供操作引導精靈幫助使用者即時除錯導引與校正提示。
提供步驟的引導教學，幫助使用者自行進行校正程序，可透過電腦或是平板來觀看校正介面，進而連線顯微鏡校正。
▲透過平板螢幕進行光路校正
▲透過除錯偵測驚嘆號，得知修正步驟
★Nikon Ti2 specifications規格表
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ECLIPSE Ti2-E / Ti2-E/B *1
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ECLIPSE T2-A
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ECLIPSE T2-U
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Main body
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Optical system
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Infinity-corrected CFI60
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Field number*2
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22 with C-mount, 25 with F-mount
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Intermediate Magnification switching
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Manual switching of 1.0x/1.5x (exchangeable from 1.5x to 2.0x)
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Status detection
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-
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Bertrand lens
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Manual in/out, and manual focus, Status detection
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-
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Output port
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4 Motorized positions
Eyepiece 100%, left 100%, right 100%,eyepiece 20%/left 80%
(Ti2-E/B: bottom 100%)
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4 Manual positions
Eyepiece 100%, left 100%, right 100%, option(to eyepiece 20%/left 80% or eyepiece 20%/right 80%)
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Can add ports by use of back port unit and/or choice of tube base unit*3
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Focusing unit
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Motorized drive, Coarse/fine focus changeover, 10mm stroke
Minimum increments: 0.01um, 0.02um (with encoder control)
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Manual drive, Coarse/fine focusing knob, 10mm stroke
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Stage up
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Available *4
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Tube body
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Binocular tube
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Binocular S tube TC-T-TS (field number 22), Ergonomic ER tube TC-T-ER (field number 22)
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Motorized eyepiece tube base unit for external PH (TI2-T-BP-E)
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Camera port (field number 16), Motorized PH turret with 4 motorized positions
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Assist eyepiece tube base unit (TI2-T-BA)
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Assist camera (field number 22), Status detection
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Eyepiece tube base unit with port (TI2-T-BC)
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Camera port (field number 16)
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Camera port(field number 16)
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Transmitted illumination
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Pillar for transmitted illumination (TI2-D-PD)
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Condenser vertical stroke: 66mm, Backward tilting up to 25 degrees, With field diagram and refocusing mechanism
2 filter slot positions (4 filter position option is also available with Filter Slider for transmitted illumination (TI2-D-SF)
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LED Lamphouse for dia illumination (TI2-D-LHLED)
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High power LED
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Precentered Lamphouse (D-LH/LC)
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100W halogen bulb (pre-centered)
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Condenser
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Motorized condenser turret (TI2-C-TC-E)
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7 motorized positions (ψ37mm x4, ψ39mm x3),
LWD/ELWD/CLWD/NAMC condenser lenses are supported
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Intelligent condenser turret (TI2-C-TC-I)
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7 manual positions (ψ37mm x4, ψ39mm x3), Status detection, LWD/ELWD/CLWD/NAMC condenser lenses are supported
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Condenser turret (TC-C-TC)
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7 manual positions (ψ37mm x4, ψ39mm x3), LWD/ELWD/CLWD/NAMC condenser lenses are supported
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ELWD-S condenser turret (TE-C)
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4 manual positions, With ELWD condenser lens (NA0.3 / OD65)
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HNA condenser slider (TI2-C-SCH)
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2 manual positions (ψ37mm x1, ψ39mm x1), HNA dry lens/ HNA oil lens are supported
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Condenser lens
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LWD (W.D.=30mm, NA=0.52), ELWD (W.D.=75mm, NA=0.3), CLWD (W.D.=13mm, NA=0.72), HNA dry (W.D.=5mm, NA=0.85), HNA oil (W.D.=1.9mm, NA=1.3), NAMC (W.D.=44mm, NA=0.4)
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Stage
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Motorized stage (TI2-S-SE-E, TI2-S-SS-E)
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Stroke X: ±57mm, Stroke Y: ±36.5mm, Max drive speed: approx. 25mm/sec, Magnetic sample holder
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Stage (TC-S-SR, TC-S-SRF)
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Stroke X: ±57mm, Stroke Y: ±36.5mm, Adjustable stroke range (3 levels) with adjusting pin, Long/middle/short handle options available
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Gliding stage (TC-S-GS)
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Stroke ψ20mm
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Nosepiece
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Perfect Focus Unit with motorized nosepiece for Auto Correction Collar (TI2-N-NDA-P)
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5 motorized positions, Simple waterproof structure
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Motorized DIC sextuple nosepiece (TI2-N-ND-E)
Perfect Focus Unit with motorized nosepiece (TI2-N-ND-P)
Perfect Focus Unit with motorized nosepiece for MP (TI2-N-NDM-P)
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6 motorized positions, Simple waterproof structure
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Intelligent DIC sextuple nosepiece (TI2-N-ND-I)
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6 manual positions, Status detection, Simple waterproof structure
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Sextuple nosepiece (TI2-N-N),
DIC sextuple nosepiece (TI2-N-ND)
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6 manual positions, Simple waterproof structure
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Epi-filter turret
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Motorized epi filter turret (TI2-F-FLT-E, TI2-F-FLTH-E)
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6 motorized positions, Motorized shutter
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Intelligent epi filter turret (TI2-F-FLT-I)
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6 manual positions, Manual shutter, Status detection*5
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Filter wheel/ shutter
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Motorized BA filter wheel (TI2-P-FWB-E)
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7 motorized positions, High speed mode: 50ms, Low vibration mode: 100ms (movement time between adjacent positions)
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Motorized shutter (NI-SH-E)*6
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12ms to open/close
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Epi-fluorescence attachment
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EPI-FL module (TI2-LA-FL),
EPI-FL module for large FOV (TI2-LA-FLL)
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Supports fiber illuminator; includes 2-position filter slider and aperture diaphragm
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Simple EPI-FL attachment (TI2-F-FLS)
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Supports both fiber illuminator and lamp house; includes 3-position filter slider
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Field stop slider
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Circular (TI2-F-FSC), rectangular (TI2-F-FSR), square (Ti2-F-FSS) aperture options
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Control unit
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Controller, display device
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Stage joystick (TI2-S-JS), Tablet
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Tablet
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Controller for TI2-E (TI2-CTRE)
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USB/LAN interface, I/O function
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Operating environmental
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Temperature: 0℃-40℃, Humidity: 60% RH max. (at +40℃, no condensation), Indoor use only
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*1 Motorized model with a bottom port
*2 Limitations apply based on objective and filter cube choice, stage-up configuration, and illumination module, etc.
*3 Tube base units with a port cannot be used with Ti2-A
*4 Stage up kit is required. Please contact Nikon.
*5 Status detection cannot be used when attached to the Ti2-U
*6 NI-SH-CON Controller for Motorized Shutter is required for use with the Ti2-A/Ti2-U
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