ORCA-Lightning
Inspiration does not obey speed limits
Features
- High resolution : 4608 x 2592 (12 Megapixels)
- High Speed: 121 frames/s (at full resolution)
- Read noise : 2.0 electrons rms (Standard Full Well Capacity mode)
- Pixel size : 5.5 μm X 5.5 μm
- Dynamic Range 17 000:1 (High Full Well Capacity mode)
- Peak QE : 60 % (at 550 nm)
高速影像擷取
- 可達到6倍於ORCA-Flash4.0 V3的速度，並且具有相似的readout noise。
- light-sheet顯微鏡要求影像快速的輸出，由於讀取速度提高的圖像導致捕獲時獲得的光子更少，因此在嘗試捕獲高S / N圖像時，有必要將相機的readout noise保持在較低水準。
- ORCA-Lightning可以完成這項困難的任務。
High speed test of 3D imaging
We took consecutive images and produced 3D imaging of a free-swimming Volvox.
When capturing the image of a free-swimming Volvox, imaging speed of 2-dimensional (area of observation) and 3-dimensional (number of images) settings were selected with a precondition of approximately 8 volumes per second.
With its high speed, ORCA-Lightning can capture clearer images within a wide range compared to ORCA-Flash4.0 V3.
Imaging conditions
- Microscope: ezDSLM (light-sheet microscope)
- Objective lens: 10×
- Excitation wavelength: 488 nm
- Fluorescence wavelength: red (autofluorescence)
- Sample: Volvox
- Observation target: Free-swimming Volvox
- Method of imaging: Acquisition of approximately 8 volume image (stereoscopic) per second
- Wide field of view(Nikon Ti2支援F.N. 25mm)
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